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Product Name
SF3B2 antibody
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Description
SF3B2 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive IF detected in HEK-293 cells. Positive WB detected in HEK-293 cells, K-562 cells. Observed molecular weight by Western-blot: 130-140 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
SAP 145 antibody; SAP145 antibody; SF3B145 antibody; SF3b150 antibody; SF3B2 antibody; Splicing factor 3B subunit 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SF3B2 recombinant protein (Accession Number: BC007610). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:10-1:100
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115137(SF3B2 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:115137(SF3B2 antibody) at dilution of 1:10 (under 40x lens)
Immunofluorescent analysis of HEK-293 cells using Catalog No:115137(SF3B2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SF3B (splicing factor 3B) is a U2 snRNP-associated protein complex essential for spliceosome assembly. SF3B complex contains the spliceosome-associated protein (SAP) 49, 130, 145, and 155 has been demonstrated. And this complex is essential for U2 snRNP to anchor to the pre-mRNA. Splicing factor 3B subunit 2 (SF3B2), known as SAP145, forms complex with SAP49 which plays a role in cell cycle progression. SAP145, with predicated MW of 100 kDa, appears with MW of about 145 kDa which may be due to the post-translational modification.
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References
- Goo YH, Cooper TA. CUGBP2 directly interacts with U2 17S snRNP components and promotes U2 snRNA binding to cardiac troponin T pre-mRNA. Nucleic acids research. 37(13):4275-86. 2009.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"