SF3A2 Polyclonal Antibody

Cat.#: 160625

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Product Information

  • Product Name
    SF3A2 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to SF3A2
  • Tested applications
    WB
  • Species reactivity
    Mouse, Rat
  • Alternative names
    SF3A2 antibody; PRP11 antibody; PRPF11 antibody; SAP62 antibody; SF3a66 antibody; splicing factor 3A subunit 2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human SF3A2 (NP_009096.2).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
  • Validations

    Western blot - SF3A2 Polyclonal Antibody

    Western blot - SF3A2 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using SF3A2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.

  • Background
    Involved in pre-mRNA splicing as a component of the splicing factor SF3A complex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex. Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes, including the Bact complex. Interacts directly with the duplex formed by U2 snRNA and the intron.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"