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Product Name
SF1 Polyclonal Antibody
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Description
Polyclonal antibody to SF1
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse
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Alternative names
SF1 antibody; BBP antibody; D11S636 antibody; MBBP antibody; ZCCHC25 antibody; ZFM1 antibody; ZNF162 antibody; splicing factor 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human SF1 (NP_973727.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - SF1 Polyclonal Antibody
Western blot analysis of extracts of HepG2 cells, using SF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - SF1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using SF1 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SF1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric cancer using SF1 Antibody at dilution of 1:100 (40x lens).
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Background
Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"