-
Product Name
SETD1B Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to SETD1B
-
Tested applications
WB, IHC, IF, IP
-
Species reactivity
Human, Mouse
-
Alternative names
SETD1B antibody; KMT2G antibody; Set1B antibody; SET domain containing 1B antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant protein of human SETD1B
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:200 - 1:2000
IHC 1:50- 1:200
IF 1:50- 1:200
IP 1:20- 1:50 -
Validations
Western blot - SETD1B Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SETD1B antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
-
Background
Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overlapping localization with SETD1A suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression. Specifically tri-methylates 'Lys-4' of histone H3 in vitro.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"