SETD1B Rabbit Polyclonal antibody. Positive WB detected in human brain tissue, MCF7 cells, mouse liver tissue. Positive IP detected in MCF-7 cells. Positive IHC detected in human breast cancer tissue, human ovary tumor tissue. Observed molecular weight by Western-blot: 250-260 kDa, 450 kDa
ELISA, WB, IP, IHC
Human, Mouse; other species not tested.
FLJ20803 antibody; hSET1B antibody; KIAA1076 antibody; KMT2G antibody; Lysine N methyltransferase 2G antibody; SET domain containing 1B antibody; Set1B antibody; SETD1B antibody
This antibody was obtained by immunization of Peptide (Accession Number: NM_015048). Purification method: Antigen affinity purified.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:115221(SETD1B antibody) at dilution of 1:800
IP Result of anti-SETD1B (IP:Catalog No:115221, 5ug; Detection:Catalog No:115221 1:300) with MCF-7 cells lysate 3250ug.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:115221(SETD1B Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using Catalog No:115221(SETD1B Antibody) at dilution of 1:50 (under 40x lens)
SETD1B, also named as KMT2G. Set1B associates with a about 450 kDa complex that contains all five non-catalytic components of the Set1A complex, including CFP1, Rbbp5, Ash2, Wdr5, and Wdr82. The complex is a histone methyltransferase that produces trimethylated histone H3 at Lys4. Set1B is widely expressed, and localize to a largely non-overlapping set of euchromatic nuclear speckles, suggesting that Set1B bind to a unique set of target genes and thus make non-redundant contributions to the epigenetic control of chromatin structure and gene expression( PMID: 17355966). The antibody is specific to SETD1B and, in westernbloting experiments, two specific bands can be detected, about 250 kDa, 450 kDa respectively, in addition, a unknown band around 70 kDa. This antibody is a rabbit polyclonal antibody raised against a region of human SETD1B.
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