NovoPro
Cart 2
  • SETD1B antibody
  • SETD1B antibody
  • SETD1B antibody
  • SETD1B antibody

SETD1B antibody

Cat.#: 115221

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    SETD1B antibody
  • Documents
  • Description
    SETD1B Rabbit Polyclonal antibody. Positive WB detected in human brain tissue, MCF7 cells, mouse liver tissue. Positive IP detected in MCF-7 cells. Positive IHC detected in human breast cancer tissue, human ovary tumor tissue. Observed molecular weight by Western-blot: 250-260 kDa, 450 kDa
  • Tested applications
    ELISA, WB, IP, IHC
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    FLJ20803 antibody; hSET1B antibody; KIAA1076 antibody; KMT2G antibody; Lysine N methyltransferase 2G antibody; SET domain containing 1B antibody; Set1B antibody; SETD1B antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Peptide (Accession Number: NM_015048). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:1000

    IHC: 1:20-1:200

  • Validations

    human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:115221(SETD1B antibody) at dilution of 1:800

    human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:115221(SETD1B antibody) at dilution of 1:800

    IP Result of anti-SETD1B (IP:Catalog No:115221, 5ug; Detection:Catalog No:115221 1:300) with MCF-7 cells lysate 3250ug.

    IP Result of anti-SETD1B (IP:Catalog No:115221, 5ug; Detection:Catalog No:115221 1:300) with MCF-7 cells lysate 3250ug.

    Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:115221(SETD1B Antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:115221(SETD1B Antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using Catalog No:115221(SETD1B Antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using Catalog No:115221(SETD1B Antibody) at dilution of 1:50 (under 40x lens)

  • Background
    SETD1B, also named as KMT2G. Set1B associates with a about 450 kDa complex that contains all five non-catalytic components of the Set1A complex, including CFP1, Rbbp5, Ash2, Wdr5, and Wdr82. The complex is a histone methyltransferase that produces trimethylated histone H3 at Lys4. Set1B is widely expressed, and localize to a largely non-overlapping set of euchromatic nuclear speckles, suggesting that Set1B bind to a unique set of target genes and thus make non-redundant contributions to the epigenetic control of chromatin structure and gene expression( PMID: 17355966). The antibody is specific to SETD1B and, in westernbloting experiments, two specific bands can be detected, about 250 kDa, 450 kDa respectively, in addition, a unknown band around 70 kDa. This antibody is a rabbit polyclonal antibody raised against a region of human SETD1B.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"