Product Name

Sestrin 2 antibody

Documents

Description

Sestrin 2 Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive IHC detected in human colon cancer tissue, human breast cancer tissue, human ovary tumor tissue. Positive IP detected in K-562 cells. Positive WB detected in HEK-293 cells, A2780 cells, HEK-293, HeLa cells, human brain tissue, human liver tissue, K-562 cells, NIH/3T3 cells, rat liver tissue, RAW264.7 cells. Observed molecular weight by Western-blot: 54-60 kDa

Tested applications

ELISA, WB, IHC, IF, IP

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

DKFZp761M0212 antibody; DKFZp761M02121 antibody; HI95 antibody; SES2 antibody; SESN2 antibody; SEST2 antibody; Sestrin 2 antibody; Sestrin-2 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of Sestrin 2 recombinant protein (Accession Number: NM_031459). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:500-1:5000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115218(Sestrin2 antibody) at dilution of 1:1000

IP Result of anti-Sestrin2 (IP:Catalog No:115218, 3ug; Detection:Catalog No:115218 1:1000) with K-562 cells lysate 4000ug.

Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:115218(Sestrin2 antibody) at dilution of 1:100 (under 25x lens)

Immunofluorescent analysis of Hela cells, using SESN2 antibody Catalog No:115218 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

WB result of Sestrin antibody (Catalog No:115218, 1:2000) with si-Control and si-Sestrin transfected HEK293 cells.

Background

SESN is a family of highly conserved, stress-inducible genes that can defend the cell against oxidative damage and oncogenic signaling. SESN2 ,which belongs to the SESN family, has been implicated as a tumor suppressor that can inhibit angiogenesis and promote autophagy, underscoring the importance of elucidating the molecular mechanism by which SESN2 regulates pathways of metabolism and survival.(PMID:22363791). Sesn2 encodes a 480 amino acid protein which can run as a 60 kDa band on SDS-polyacrylamide gel electrophoresis(PMID:20878915).

References

Yang Y, Cuevas S, Yang S. Sestrin2 decreases renal oxidative stress, lowers blood pressure, and mediates dopamine D2 receptor-induced inhibition of reactive oxygen species production. Hypertension. 64(4):825-32. 2014.
Zhao B, Shah P, Budanov AV. Sestrin2 protein positively regulates AKT enzyme signaling and survival in human squamous cell carcinoma and melanoma cells. The Journal of biological chemistry. 289(52):35806-14. 2014.
Tomasovic A, Kurrle N, Sürün D. Sestrin 2 protein regulates platelet-derived growth factor receptor β (Pdgfrβ) expression by modulating proteasomal and Nrf2 transcription factor functions. The Journal of biological chemistry. 290(15):9738-52. 2015.
Yang JH, Kim KM, Kim MG. Role of sestrin2 in the regulation of proinflammatory signaling in macrophages. Free radical biology & medicine. 78:156-67. 2015.
Seo K, Ki SH, Shin SM. Sestrin2-AMPK activation protects mitochondrial function against glucose deprivation-induced cytotoxicity. Cellular signalling. 27(7):1533-43. 2015.
Ding B, Parmigiani A, Yang C, Budanov AV. Sestrin2 facilitates death receptor-induced apoptosis in lung adenocarcinoma cells through regulation of XIAP degradation. Cell cycle (Georgetown, Tex.). 14(20):3231-41. 2015.
Ye J, Palm W, Peng M. GCN2 sustains mTORC1 suppression upon amino acid deprivation by inducing Sestrin2. Genes & development. 29(22):2331-6. 2015.
Ben-Sahra I, Dirat B, Laurent K. Sestrin2 integrates Akt and mTOR signaling to protect cells against energetic stress-induced death. Cell death and differentiation. 20(4):611-9. 2013.

Sestrin 2 antibody

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