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Product Name
SERPINH1 Polyclonal Antibody
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Description
Polyclonal antibody to SERPINH1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse
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Alternative names
SERPINH1 antibody; AsTP3 antibody; CBP1 antibody; CBP2 antibody; HSP47 antibody; OI10 antibody; PIG14 antibody; PPROM antibody; RA-A47 antibody; SERPINH2 antibody; gp46 antibody; serpin H1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 139-418 of human SERPINH1 (NP_001226.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - SERPINH1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SERPINH1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - SERPINH1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using SERPINH1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - SERPINH1 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using SERPINH1 antibody . Blue: DAPI for nuclear staining.
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Background
Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"