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Product Name
SERPINA4 Polyclonal Antibody
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Description
Polyclonal antibody to SERPINA4
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
SERPINA4 antibody; KAL antibody; KLST antibody; KST antibody; PI-4 antibody; PI4 antibody; kallistatin antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 178-427 of human SERPINA4 (NP_006206.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100 -
Validations
Western blot - SERPINA4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SERPINA4 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Immunohistochemistry - SERPINA4 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using SERPINA4 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - SERPINA4 Polyclonal Antibody
Immunofluorescence analysis of MCF7 cells using SERPINA4 antibody . Blue: DAPI for nuclear staining.
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Background
Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"