Human TLK2/PKU-ALPHA (aa 397-772, His & GST Tag) recombinant protein

Human TLK2/PKU-ALPHA (aa 397-772, His & GST Tag) recombinant protein

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Serine / threonine-protein kinase tousled-like 2, also known as PKU-alpha, Tousled-like kinase 2 and TLK2, is a nucleus protein which belongs to the protein kinase superfamily and Ser/Thr protein kinase family. The tousled-like kinases are an evolutionarily conserved family of proteins implicated in DNA repair, DNA replication and mitosis in metazoans and plants. Their absence from the yeasts and other eukaryotic 'microbes' suggests a specific role for them in the development of multicellular organisms. Tousled-like kinase 2 / TLK2 is widely expressed. It is present in fetal placenta, liver, kidney, pancreas, heart and skeletal muscle. It is also found in adult cell lines. Tousled-like kinase 2 / TLK2 contains one protein kinase domain. Tousled-like kinase 2 / TLK2 is rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly.

Serine/threonine-protein kinase tousled-like 2

TLK; PKUALPHA; PKU-ALPHA; TLK2; MGC44450; HSHPK; 4933403M19RIK; RP23-456M3.2

Q86UE8

TLK2

Baculovirus-Insect Cells

A DNA sequence encoding the amino acids (Leu 397-Asn 772) of human TLK2 (Q86UE8-1) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Human

The recombinant human TLK2 (aa 397-772)/GST chimera consists of 613 amino acids and has a calculated molecular mass of 71.3 kDa. It migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Kinase activity untested