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Product Name
Human ERN1/IRE1 (aa 465-977) recombinant protein
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Description
Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
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Protein name
Serine/threonine-protein kinase/endoribonuclease IRE1
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Protein short names
IRE1ALPHA; IRE1A; MGC163277; HIRE1P; MGC163279; 9030414B18RIK; C85377; IRE1P; RP23-418O11.11-001; FLJ30999; AI225830; IRE1; ERN1
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Uniprot ID
O75460
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Gene Name
ERN1; IRE1
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Source/Expression Host
Baculovirus-Insect Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human ERN1 (O75460-1) (Pro 465-Leu 977) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
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Protein Species
Human
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Molecular weight
The secreted recombinant human ERN1 consists of 515 amino acids and predicts a molecular mass of 58.3 KDa. The apparent molecular mass of the protein is approximately 65 KDa in SDS-PAGE under reducing conditions due to glycosylation.
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Purity
> 80 % as determined by SDS-PAGE
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Activity
1. Kinase activity untested
2. Measured by its nuclease activity to cleave Xbp1 single stem-loop mini-substrate. -
Validations
Human ERN1 / IRE1 Protein (aa 465-977) SDS-PAGE
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