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Product Name
Anti-ERN1/IRE1 antibody
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Description
Mouse monoclonal to ERN1/IRE1
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Tested applications
ELISA, IHC-P
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Species reactivity
Human ERN1 / IRE1
No cross-reactivity in ELISA with Insect cell lysate -
Alternative names
Ire1p antibody; IRE1 antibody; IRE1P antibody; IRE1a antibody; hIRE1p antibody; Ire1a antibody; Ire1p antibody; C85377 antibody; AI225830 antibody; Ire1alpha antibody; 9030414B18Rik antibody; 9030414B18Rik antibody; AI225830 antibody; C85377 antibody; ERN1 antibody; Ern1 antibody; FLJ30999 antibody; hIRE1p antibody; IRE1 antibody; Ire1a antibody; IRE1a antibody; Ire1alpha antibody; IRE1P antibody; MGC163277 antibody; MGC163279 antibody; RP23-418O11.11-001 antibody
- Immunogen
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Isotype
Mouse IgG2b
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ERN1 / IRE1 (rh ERN1 / IRE1; O75460-1; Pro 465-Leu 977). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human ERN1 / IRE1. The detection limit for Human ERN1 / IRE1 is approximately 0.31 ng/well.
IHC-P: 1-20 μg/mL
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Validations
ERN1 / IRE1 Antibody, Mouse MAb
Immunochemical staining of human ERN1 in human liver with mouse monoclonal antibody (3 µg/mL, formalin-fixed paraffin embedded sections).
ERN1 / IRE1 Antibody, Mouse MAb
Immunochemical staining of human ERN1 in human tonsil with mouse monoclonal antibody (3 µg/mL, formalin-fixed paraffin embedded sections).
ERN1 / IRE1 Antibody, Mouse MAb
Immunochemical staining of human ERN1 in human epididymis with mouse monoclonal antibody (10 µg/mL, formalin-fixed paraffin embedded sections).
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References
- Trentmann,S.M. et al., 2000, Plant Mol Biol. 44 (1):11-25.
- Iwawaki T., et al., 2001, Nat. Cell Biol. 3:158-64.
- Liu C.Y., et al., 2003, J. Biol. Chem. 278:17680-7.
- Oppermann F.S., et al., 2009, Mol. Cell. Proteomics 8:1751-64.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"