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Product Name
SEPT7 antibody
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Description
SEPT7 Rabbit Polyclonal antibody. Positive IF detected in hTERT-RPE cells, HepG2 cells. Positive IHC detected in human lung cancer tissue, human gliomas tissue. Positive WB detected in HepG2 cells, A375 cells, HeLa cells, Jurkat cells, mouse testis tissue, rat testis tissue. Observed molecular weight by Western-blot: 45-50 kDa
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Tested applications
ELISA, IHC, IF, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CDC10 antibody; CDC10 protein homolog antibody; CDC3 antibody; Nbla02942 antibody; SEPT7 antibody; SEPT7A antibody; septin 7 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SEPT7 recombinant protein (Accession Number: BC025987). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:115121(SEPT7 antibody) at dilution of 1:2000
IF result of anti-SEPT7(Catalog No:115121, 1:1000) with hTERT-RPE cell by Dr. Moshe Kim. Green: SEPT7. Blue: DNA. 1% PFA fixed
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:115121(SEPT7 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:115121(SEPT7 antibody) at dilution of 1:100 (under 40x lens)
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Background
SEPT7 represents septin 7
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References
- Phan QT, Eng DK, Mostowy S, Park H, Cossart P, Filler SG. Role of endothelial cell septin 7 in the endocytosis of Candida albicans. mBio. 4(6):e00542-13. 2013.
- Kim MS, Froese CD, Xie H, Trimble WS. Uncovering principles that control septin-septin interactions. The Journal of biological chemistry. 287(36):30406-13. 2012.
- Gera N, Yang A, Holtzman TS, Lee SX, Wong ET, Swanson KD. Tumor treating fields perturb the localization of septins and cause aberrant mitotic exit. PloS one. 10(5):e0125269. 2015.
- Calvo F, Ranftl R, Hooper S. Cdc42EP3/BORG2 and Septin Network Enables Mechano-transduction and the Emergence of Cancer-Associated Fibroblasts. Cell reports. 13(12):2699-714. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"