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Product Name
SEPT11 antibody
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Description
SEPT11 Rabbit Polyclonal antibody. Positive IHC detected in human ovary tissue, human kidney tissue, human lung tissue, human spleen tissue. Positive IF detected in Hela cells, HepG2 cells. Positive IP detected in mouse brain tissue. Positive WB detected in human brain tissue, HeLa cells, HT-1080 cells, mouse brain tissue, mouse colon tissue, mouse small intestine tissue, SH-SY5Y cells. Observed molecular weight by Western-blot: 48 kDa
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Tested applications
ELISA, WB, IF, IP, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
SEPT11 antibody; septin 11 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SEPT11 recombinant protein (Accession Number: NM_018243). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:115115(SEPT11 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human ovary using Catalog No:115115(SEPT11 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human ovary using Catalog No:115115(SEPT11 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using SEPT11 antibody Catalog No:115115 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-SEPT11 (IP:Catalog No:115115, 4ug; Detection:Catalog No:115115 1:500) with mouse brain tissue lysate 3440ug.
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Background
SEPT11 represents septin 11
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