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Product Name
SEMA7A Polyclonal Antibody
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Description
Polyclonal antibody to SEMA7A
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Tested applications
WB
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Species reactivity
Human
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Alternative names
SEMA7A antibody; CD108 antibody; CDw108 antibody; H-SEMA-K1 antibody; H-Sema-L antibody; JMH antibody; SEMAK1 antibody; SEMAL antibody; semaphorin-7A antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 45-280 of human SEMA7A (NP_003603.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
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Validations
Western blot - SEMA7A Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SEMA7A antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
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Background
Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"