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Product Name
Seladin 1 antibody
- Documents
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Description
Seladin 1 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, HEK-293 cells, HepG2 cells, MCF7 cells, mouse lung tissue. Positive IHC detected in human breast cancer tissue, human liver tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue. Observed molecular weight by Western-blot: 60kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DHCR24 antibody; Diminuto/dwarf1 homolog antibody; KIAA0018 antibody; Nbla03646 antibody; Seladin 1 antibody; SELADIN1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Seladin 1 recombinant protein (Accession Number: NM_014762). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:115099(DHCR24 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:115099(DHCR24 antibody) at dilution of 1:100 (under 25x lens)
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Background
DHCR24(3-beta-hydroxysterol delta-24-reductase), a member of the flavin adenine dinucleotide (FAD)-dependent oxidoreductases, catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis.The dhcr24 expression was shown to be downregulated in brain areas affected by Alzheimer’s disease and endogenous DHCR24/seladin-1 levels are highly up-regulated upon acute oxidative stress while expression declines to very low levels upon chronic exposure(PMID:17984220).
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References
- Khuda II, Koide N, Noman AS. Seladin-1 is a novel lipopolysaccharide (LPS)-responsive gene and inhibits the tumour necrosis factor-alpha production and osteoclast formation in response to LPS. Immunology. 131(1):59-66. 2010.
- Mirza R, Qiao S, Tateyama K, Miyamoto T, Xiuli L, Seo H. 3β-Hydroxysterol-Delta24 reductase plays an important role in long bone growth by protecting chondrocytes from reactive oxygen species. Journal of bone and mineral metabolism. 30(2):144-53. 2012.
- Legg RL, Tolman JR, Lovinger CT, Lephart ED, Setchell KD, Christensen MJ. Diets high in selenium and isoflavones decrease androgen-regulated gene expression in healthy rat dorsolateral prostate. Reproductive biology and endocrinology : RB&E. 6:57. 2008.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"