Human SFRP1 (His Tag) recombinant protein

Human SFRP1 (His Tag) recombinant protein

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Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription.

Secreted frizzled-related protein 1

AW107218; SFRP1; FRZA; FRP1; AW742929; FRP; SFRP-1; SARP2; 2210415K03RIK; FRP-1; AW011917

Q8N474

SFRP1; FRP; FRP1; SARP2

Human Cells

A DNA sequence encoding the human sFRP1 (NP_003003.3) (Met 1-Lys 314) was expressed with a C-terminal polyhistidine tag.

Human

The recombinant human sFRP1 consists of 294 amino acids after removal of the signal peptide and has a predicted molecular mass of 34 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 38 kDa due to glycosylation.

> 97 % as determined by SDS-PAGE

Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED₅₀ for this effect is typically 2.5-10 μg/ml.