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Product Name
Anti-SFRP1 antibody
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Description
Rabbit polyclonal antibody to SFRP1
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Tested applications
WB, ICC, IHC-P, FC
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Species reactivity
Human, Mouse, Rat
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Alternative names
FRP antibody; FRP1 antibody; FrzA antibody; FRP-1 antibody; SARP2 antibody
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Isotype
Rabbit IgG
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Preparation
This antigen of this antibody was peptide
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Clonality
Polyclonal
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Formulation
Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:500-1:1,000
ICC: 1:100-1:500
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Validations
Fig1: Western blot analysis of SFRP1 on SH-SY-5Y cells lysates using anti-SFRP1 antibody at 1/500 dilution.
Fig2: ICC staining SFRP1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining SFRP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-SFRP1 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SFRP1 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-SFRP1 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-SFRP1 antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-SFRP1 antibody. Counter stained with hematoxylin.
Fig9: Flow cytometric analysis of SH-SY-5Y cells with SFRP1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secon
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