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Product Name
SEC61B-Specific antibody
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Description
SEC61B-Specific Rabbit Polyclonal antibody. Positive IHC detected in human cervical cancer tissue, human liver tissue. Positive IF detected in HeLa cells. Positive WB detected in mouse liver tissue, HeLa cells, human kidney tissue. Observed molecular weight by Western-blot: 10 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
Sec61 beta subunit antibody; SEC61B antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_006808). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:115088(SEC61B-Specific antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:115088(SEC61B-Specific Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:115088(SEC61B-Specific Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:115088(SEC61B-Specific Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
The heteromeric SEC61 complex is composed of alpha, beta and gamma subunits. Oligomers of the SEC61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane (PMID: 10212142). SEC61B is the beta subunit of the SEC61 complex. It has been reported that SEC61B facilitates cotranslational protein transport and interacts with the signal peptidase during translocation (PMID: 9585408).
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