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Product Name
SEC61alpha antibody
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Description
SEC61alpha Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in mouse brain tissue, HEK-293 cells, mouse liver tissue. Observed molecular weight by Western-blot: 52 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
FLJ10578 antibody; Sec61 alpha 2 antibody; SEC61A antibody; SEC61A2 antibody; SEC61alpha antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SEC61alpha recombinant protein (Accession Number: BC026179). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:115084(SEC61A2 Antibody) at dilution of 1:600
Immunofluorescent analysis of HepG2 cells using Catalog No:115084(SEC61A2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
The SEC61 complex is a major component of the endoplasmic reticulum (ER) translocation site (PMID: 7720858). This complex is composed of three subunits: SEC61alpha, SEC61beta and SEC61gamma. SEC61alpha is a polytopic integral membrane protein. It is a core component of the ER translocation complex, playing a crucial role in the insertion of secretory and membrane polypeptides into the ER (PMID: 9512475).
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