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Product Name
SEC5/EXOC2 antibody
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Description
SEC5/EXOC2 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive WB detected in mouse brain tissue, human brain tissue, human ileum tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 95-100 kDa
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Tested applications
ELISA, IHC, IP, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
EXOC2 antibody; exOCyst complex component 2 antibody; ExOCyst complex component Sec5 antibody; FLJ11026 antibody; SEC5 antibody; SEC5L1 antibody; Sec5p antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SEC5/EX℃2 recombinant protein (Accession Number: BC016918). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:115082(SEC5 antibody) at dilution of 1:500
IP Result of anti-SEC5 (IP:Catalog No:115082, 5ug; Detection:Catalog No:115082 1:500) with mouse brain tissue lysate 3000ug.
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:115082(SEC5 antibody) at dilution of 1:100 (under 10x lens)
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Background
EXoc2 (exocyst complex component 2), also known as SEC5 and SEC5L1, is a component of the exocyst complex, and is required to mediate RalB-dependent survival signals in transformed cells. The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is involved in tethering post-Golgi secretory vesicles to specific plasma membrane domains. The gene of EXoc2 maps to chromosome 6p25.3, and encodes a 924-amino acid protein with an experimentally determined molecular mass of 95-100 kDa. EXoc2 mRNA is widely expressed with highest levels in brain and placenta.
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References
- Mukerji J, Olivieri KC, Misra V, Agopian KA, Gabuzda D. Proteomic analysis of HIV-1 Nef cellular binding partners reveals a role for exocyst complex proteins in mediating enhancement of intercellular nanotube formation. Retrovirology. 9:33. 2012.
- Takeuchi H, Furuta N, Morisaki I, Amano A. Exit of intracellular Porphyromonas gingivalis from gingival epithelial cells is mediated by endocytic recycling pathway. Cellular microbiology. 13(5):677-91. 2011.
- Sánchez-Ruiz J, Mejías R, García-Belando M, Barber DF, González-García A. Ral GTPases regulate cell-mediated cytotoxicity in NK cells. Journal of immunology (Baltimore, Md. : 1950). 187(5):2433-41. 2011.
- Hase K, Kimura S, Takatsu H. M-Sec promotes membrane nanotube formation by interacting with Ral and the exocyst complex. Nature cell biology. 11(12):1427-32. 2009.
- Simicek M, Lievens S, Laga M. The deubiquitylase USP33 discriminates between RALB functions in autophagy and innate immune response. Nature cell biology. 15(10):1220-30. 2013.
- Tan X, Thapa N, Sun Y, Anderson RA. A kinase-independent role for EGF receptor in autophagy initiation. Cell. 160(1-2):145-60. 2015.
- Takeuchi H, Takada A, Kuboniwa M, Amano A. Intracellular periodontal pathogen exploits recycling pathway to exit from infected cells. Cellular microbiology. 2015.
- Rauch L, Hennings K, Aepfelbacher M. A role for exocyst in maturation and bactericidal function of staphylococci-containing endothelial cell phagosomes. Traffic (Copenhagen, Denmark). 15(10):1083-98. 2014.
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