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Product Name
SEC24A antibody
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Description
SEC24A Rabbit Polyclonal antibody. Positive WB detected in human liver tissue, mouse skeletal muscle tissue, mouse thymus tissue, rat thymus tissue. Positive IF detected in Hela cells. Positive IHC detected in human lymphoma tissue. Observed molecular weight by Western-blot: 66 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
SEC24 related protein A antibody; SEC24A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SEC24A recombinant protein (Accession Number: NM_001252231). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:115079(SEC24A antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:115079(SEC24A antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:115079(SEC24A antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using SEC24A antibody Catalog No:115079 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).
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References
- Taguchi Y, Imaoka K, Kataoka M. Yip1A, a novel host factor for the activation of the IRE1 pathway of the unfolded protein response during Brucella infection. PLoS pathogens. 11(3):e1004747. 2015.
- Han J, Li E, Chen L. The CREB coactivator CRTC2 controls hepatic lipid metabolism by regulating SREBP1. Nature. 524(7564):243-6. 2015.
- Gustafsen C, Kjolby M, Nyegaard M. The hypercholesterolemia-risk gene SORT1 facilitates PCSK9 secretion. Cell metabolism. 19(2):310-8. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"