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Product Name
SDHD Polyclonal Antibody
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Description
Polyclonal antibody to SDHD
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Tested applications
WB
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Species reactivity
Mouse
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Alternative names
SDHD antibody; CBT1 antibody; CII-4 antibody; CWS3 antibody; PGL antibody; PGL1 antibody; QPs3 antibody; SDH4 antibody; cybS antibody; succinate dehydrogenase complex subunit D antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human SDHD (NP_002993.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - SDHD Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SDHD antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
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Background
Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"