-
Product Name
SDHB antibody
- Documents
-
Description
SDHB Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells. Positive IHC detected in human heart tissue. Observed molecular weight by Western-blot: 32 kDa
-
Tested applications
ELISA, WB, IHC, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
FLJ92337 antibody; PGL4 antibody; SDH antibody; SDH1 antibody; SDHB antibody; SDHIP antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of SDHB recombinant protein (Accession Number: NM_003000). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
-
Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115032(SDHB antibody) at dilution of 1:1000
IP Result of anti-SDHB (IP:Catalog No:115032, 4ug; Detection:Catalog No:115032 1:500) with HEK-293 cells lysate 2800ug.
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:115032(SDHB Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:115032(SDHB Antibody) at dilution of 1:50 (under 40x lens)
-
Background
SDHB(Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial) is also named as SDH, SDH1 and belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. The SDHD, SDHB, and SDHC genes encode subunits of mitochondrial complex II (succinate dehydrogenase).Mitochondrial complex II is a heterotetrameric complex involved in the aerobic electron transport chain and catalyses the oxidation of succinate to fumarate (Krebs cycle) with transport of electrons to the ubiquinone pool. SDHA and SDHB are the hydrophilic catalytic part of the complex and are highly conserved(J Med Genet 2004;41:e99). Defects in SDHB are a cause of susceptibility to pheochromocytoma (PCC), paragangliomas type 4 (PGL4), paraganglioma and gastric stromal sarcoma (PGGSS) and Cowden-like syndrome (CWDLS).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"