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Product Name
SDHA antibody
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Description
SDHA Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue. Positive IP detected in mouse brain tissue. Positive IHC detected in human kidney tissue, human heart tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 62-66 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
SDH2 antibody; SDHA antibody; SDHF antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SDHA recombinant protein (Accession Number: NM_004168). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115031(SDHA antibody) at dilution of 1:800
IP Result of anti-SDHA (IP:Catalog No:115031, 4ug; Detection:Catalog No:115031 1:500) with mouse brain tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:115031(SDHA Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:115031(SDHA Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:115031(SDHA Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SDHA, also named as SDH2, SDHF and Fp, belongs to the FAD-dependent oxidoreductase 2 family and FRD/SDH subfamily. SDHA subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). It can be detected a band of 68 kDa in cutaneous melanoma(PMID:21698147). It is act as a tumor suppressor.
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References
- Ma X, Li C, Sun L. Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1. Nature communications. 5:5212. 2014.
- Li H, Ruan Y, Zhang K. Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization. Cell death and differentiation. 23(3):380-92. 2016.
- Zhang J, Wang C, Chen X. EglN2 associates with the NRF1-PGC1α complex and controls mitochondrial function in breast cancer. The EMBO journal. 34(23):2953-70. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"