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Product Name
SDCCAG8 antibody
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Description
SDCCAG8 Rabbit Polyclonal antibody. Positive IF detected in MDCK cells, Hela cells. Positive WB detected in PC-3 cells, COLO 320 cells, mouse thymus tissue, Transfected HEK-293 cells. Observed molecular weight by Western-blot: 57 kDa,63 kDa,41-45 kDa,73-83 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat,Zebrafish; other species not tested.
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Alternative names
Antigen NY CO 8 antibody; BBS16 antibody; CCCAP antibody; hCCCAP antibody; HSPC085 antibody; NY CO 8 antibody; SDCCAG8 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SDCCAG8 recombinant protein (Accession Number: XM_005273023). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:20-1:200
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:115028(SDCCAG8 antibody) at dilution of 1:500
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115028(SDCCAG8 Antibody) at dilution of 1:500
Immunofluorescent analysis of MDCK cells using Catalog No:115028(SDCCAG8 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of MDCK cells using Catalog No:115028(SDCCAG8 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SDCCAG8, also named as CCCAP and NY-CO-8, plays a role in the establishment of cell polarity and epithelial lumen formation. It may play a role in ciliogenesis. Loss of SDCCAG8 function as a cause of a retinal-renal ciliopathy and validates exome capture analysis for broadly heterogeneous single-gene disorders. SDCCAG8 is localized at both centrioles and interacts directly with OFD1 (oral-facial-digital syndrome 1), which is associated with NPHP-RC.
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References
- Airik R, Slaats GG, Guo Z. Renal-retinal ciliopathy gene Sdccag8 regulates DNA damage response signaling. Journal of the American Society of Nephrology : JASN. 25(11):2573-83. 2014.
- Otto EA, Hurd TW, Airik R. Candidate exome capture identifies mutation of SDCCAG8 as the cause of a retinal-renal ciliopathy. Nature genetics. 42(10):840-50. 2010.
- Patil H, Tserentsoodol N, Saha A, Hao Y, Webb M, Ferreira PA. Selective loss of RPGRIP1-dependent ciliary targeting of NPHP4, RPGR and SDCCAG8 underlies the degeneration of photoreceptor neurons. Cell death & disease. 3:e355. 2012.
- Jäger S, Cimermancic P, Gulbahce N. Global landscape of HIV-human protein complexes. Nature. 481(7381):365-70. 2012.
- Lee YL, Santé J, Comerci CJ. Cby1 promotes Ahi1 recruitment to a ring-shaped domain at the centriole-cilium interface and facilitates proper cilium formation and function. Molecular biology of the cell. 25(19):2919-33. 2014.
- Insolera R, Shao W, Airik R, Hildebrandt F, Shi SH. SDCCAG8 regulates pericentriolar material recruitment and neuronal migration in the developing cortex. Neuron. 83(4):805-22. 2014.
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