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Product Name
SCYL1 Polyclonal Antibody
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Description
Polyclonal antibody to SCYL1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
SCYL1 antibody; GKLP antibody; HT019 antibody; NKTL antibody; NTKL antibody; P105 antibody; SCAR21 antibody; TAPK antibody; TEIF antibody; TRAP antibody; SCY1 like pseudokinase 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 460-740 of human SCYL1 (NP_065731.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100 -
Validations
Western blot - SCYL1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SCYL1 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunohistochemistry - SCYL1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver injury using SCYL1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - SCYL1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using SCYL1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - SCYL1 Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using SCYL1 antibody . Blue: DAPI for nuclear staining.
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Background
Regulates COPI-mediated retrograde protein traffic at the interface between the Golgi apparatus and the endoplasmic reticulum. Involved in the maintenance of the Golgi apparatus morphology. Has no detectable kinase activity in vitro.; Isoform 6 acts as transcriptional activator. It binds to three different types of GC-rich DNA binding sites (box-A, -B and -C) in the beta-polymerase promoter region. It also binds to the TERT promoter region.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"