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Product Name
SCNN1G Polyclonal Antibody
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Description
Polyclonal antibody to SCNN1G
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse
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Alternative names
SCNN1G antibody; BESC3 antibody; ENaCg antibody; ENaCgamma antibody; PHA1 antibody; SCNEG antibody; sodium channel epithelial 1 gamma subunit antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 85-355 of human SCNN1G (NP_001030.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:50 - 1:100 -
Validations
Western blot - SCNN1G Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SCNN1G antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 60s.
Immunohistochemistry - SCNN1G Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using SCNN1G antibody at dilution of 1:100 (40x lens).
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Background
Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"