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Product Name
SCNN1A Polyclonal Antibody
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Description
Polyclonal antibody to SCNN1A
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
SCNN1A antibody; BESC2 antibody; ENaCa antibody; ENaCalpha antibody; SCNEA antibody; SCNN1 antibody; sodium channel epithelial 1 alpha subunit antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 105-225 of human SCNN1A (NP_001029.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - SCNN1A Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SCNN1A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
Immunofluorescence - SCNN1A Polyclonal Antibody
Immunofluorescence analysis of human lung cancer cells using SCNN1A antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - SCNN1A Polyclonal Antibody
Immunofluorescence analysis of rat lung cells using SCNN1A antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - SCNN1A Polyclonal Antibody
Immunofluorescence analysis of mouse kidney cells using SCNN1A antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - SCNN1A Polyclonal Antibody
Immunofluorescence analysis of human lung cancer cells using SCNN1A antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - SCNN1A Polyclonal Antibody
Immunofluorescence analysis of rat lung cells using SCNN1A antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and eccrine sweat glands. Also plays a role in taste perception.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"