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Product Name
SART1 Polyclonal Antibody
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Description
Polyclonal antibody to SART1
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Tested applications
WB, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
SART1 antibody; Ara1 antibody; HOMS1 antibody; SART1259 antibody; SNRNP110 antibody; Snu66 antibody; HAF antibody; U4/U6.U5 tri-snRNP-associated protein 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human SART1 (NP_005137.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IP 1:20 - 1:50 -
Validations
Western blot - SART1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using SART1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunoprecipitation - SART1 Polyclonal Antibody
Immunoprecipitation analysis of 150ug extracts of Jurkat cells using 3ug SART1 antibody . Western blot was performed from the immunoprecipitate using SART1 antibody at a dilition of 1:1000.
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Background
Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"