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Product Name
SART1 antibody
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Description
SART1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, Jurkat cells. Positive IP detected in mouse brain tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 110 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
Ara1 antibody; Hom s 1 antibody; HOMS1 antibody; hSART 1 antibody; hSnu66 antibody; SART 1 antibody; SART1 antibody; SART1259 antibody; SNRNP110 antibody; Snu66 antibody; SNU66 homolog antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SART1 recombinant protein (Accession Number: NM_005146). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114972(SART1 antibody) at dilution of 1:1000
Immunofluorescent analysis of HepG2 cells, using SART1 antibody Catalog No:114972 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-SART1 (IP:Catalog No:114972, 4ug; Detection:Catalog No:114972 1:1000) with mouse brain tissue lysate 5200ug.
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Background
Squamous cell carcinoma antigen recognized by T cells (SART1), also known as U4/U6.U5 tri-snRNP-associated 110 kDa protein, is a ubiquitously expressed protein that is involved in mRNA splicing and the regulation of cell proliferation. It has been identified as regulator of c-FLIP and drug-induced activation of caspase 8. The SART1 gene encodes two proteins, the SART1(800) protein is located in the nucleus of the majority of proliferating cells, while the SART1(259) protein is located in the cytosol of epithelial cancers. Genetic variation in the SART1 gene may be associated with breast cancer development. This antibody recognizes the endogenous 800-amino acid SART1, which migrates with an apparent molecular mass of 110 kDa in SDS-PAGE. (PMID: 22027693; 9449708; 19377877; 11350945)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"