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Product Name
SAP18 antibody
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Description
SAP18 Rabbit Polyclonal antibody. Positive IP detected in K-562 cells. Positive WB detected in K-562 cells. Positive IF detected in Hela cells. Positive IHC detected in human cervical cancer tissue. Observed molecular weight by Western-blot: 18 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
2HOR0202 antibody; SAP18 antibody; SAP18P antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SAP18 recombinant protein (Accession Number: BC030836). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:115060(SAP18 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:115060(SAP18 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:115060(SAP18 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using SAP18 antibody Catalog No:115060 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-SAP18 (IP:Catalog No:115060, 4ug; Detection:Catalog No:115060 1:500) with K-562 cells lysate 3200ug.
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Background
SAP18 is part of the SIN3-repressing complex, which enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism
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