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SAMHD1 antibody

Cat.#: 115056

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Product Information

  • Product Name
    SAMHD1 antibody
  • Documents
  • Description
    SAMHD1 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, human brain tissue, human heart tissue, K-562 cells, L02 cells, mouse heart tissue. Positive IP detected in K-562 cells. Positive IHC detected in human lung cancer tissue, human colon cancer tissue. Positive IF detected in HepG2 cells. Positive FC detected in K-562 cells. Observed molecular weight by Western-blot: 64-72 kDa
  • Tested applications
    ELISA, WB, IHC, IP, FC, IF
  • Species reactivity
    Human, Mouse, Rat; other species not tested.
  • Alternative names
    DCIP antibody; HDDC1 antibody; MonOCyte protein 5 antibody; MOP 5 antibody; MOP5 antibody; SAM domain and HD domain 1 antibody; SAMHD1 antibody; SBBI88 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of SAMHD1 recombinant protein (Accession Number: NM_015474). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:1000

    IHC: 1:20-1:200

    IF: 1:50-1:500

  • Validations

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:115056(SAMHD1 antibody) at dilution of 1:1000

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:115056(SAMHD1 antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:115056(SAMHD1 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:115056(SAMHD1 antibody) at dilution of 1:50 (under 40x lens)

    IP Result of anti-SAMHD1 (IP:Catalog No:115056, 3ug; Detection:Catalog No:115056 1:300) with K-562 cells lysate 2400ug.

    IP Result of anti-SAMHD1 (IP:Catalog No:115056, 3ug; Detection:Catalog No:115056 1:300) with K-562 cells lysate 2400ug.

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:115056(SAMHD1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:115056(SAMHD1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    1X10^6 K-562 cells were stained with 0.2ug SAMHD1 antibody (Catalog No:115056, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 K-562 cells were stained with 0.2ug SAMHD1 antibody (Catalog No:115056, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    SAMHD1, highly expressed by monocytes and monocyte-derived dendritic cells and by monocyte-derived macrophages at a lower extent, is reported as an HIV-1 restriction factor that inhibits the early step of the HIV-1 life cycle. Vpx (virus-like particles containing viral protein X) could overcome this block by interacting with SAMHD1, inducing proteasome-dependent degradation of SAMHD1. Mutations in SAMHD1 cause Aicardi-Goutières syndrome, a genetic encephalopathy with a presumed immune pathogenesis. This antibody 12586-1-AP is raised againt N-terminal 314 amino acids of the full length SAMHD1.
  • References
    • Kretschmer S, Wolf C, König N. SAMHD1 prevents autoimmunity by maintaining genome stability. Annals of the rheumatic diseases. 74(3):e17. 2015.
    • Baldauf HM, Pan X, Erikson E. SAMHD1 restricts HIV-1 infection in resting CD4(+) T cells. Nature medicine. 18(11):1682-7. 2012.
    • Reinhard C, Bottinelli D, Kim B, Luban J. Vpx rescue of HIV-1 from the antiviral state in mature dendritic cells is independent of the intracellular deoxynucleotide concentration. Retrovirology. 11:12. 2014.
    • Ballana E, Badia R, Terradas G. SAMHD1 specifically affects the antiviral potency of thymidine analog HIV reverse transcriptase inhibitors. Antimicrobial agents and chemotherapy. 58(8):4804-13. 2014.
    • Chen Z, Zhu M, Pan X. Inhibition of Hepatitis B virus replication by SAMHD1. Biochemical and biophysical research communications. 450(4):1462-8. 2014.
    • Zhou T, Dang Y, Baker JJ, Zhou J, Zheng YH. Evidence for Vpr-dependent HIV-1 replication in human CD4+ CEM.NKR T-cells. Retrovirology. 9:93. 2012.
    • Puigdomènech I, Casartelli N, Porrot F, Schwartz O. SAMHD1 restricts HIV-1 cell-to-cell transmission and limits immune detection in monocyte-derived dendritic cells. Journal of virology. 87(5):2846-56. 2013.
    • Rice GI, Bond J, Asipu A. Mutations involved in Aicardi-Goutières syndrome implicate SAMHD1 as regulator of the innate immune response. Nature genetics. 41(7):829-32. 2009.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"