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Product Name
SAFB2 antibody
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Description
SAFB2 Rabbit Polyclonal antibody. Positive WB detected in human brain tissue, BxPC-3 cells, HeLa cells, MCF7 cells, rat pancreas tissue. Positive IHC detected in human breast cancer tissue. Observed molecular weight by Western-blot: 130 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
KIAA0138 antibody; SAF B2 antibody; SAFB2 antibody; scaffold attachment factor B2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SAFB2 recombinant protein (Accession Number: BC025279). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:115044(SAFB2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:115044(SAFB2 antibody) at dilution of 1:50 (under 40x lens)
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Background
SAFB2, also named as Scaffold attachment factor B2 or KIAA0138, is a 953 amino acid protein, which contains one SAP domain and one RRM (RNA recognition motif) domain. SAFB2 expressed at high levels in the CNS and at low levels in the liver. It also is expressed in a wide number of breast cancer cell lines. SAFB2 binds to scaffold/matrix attachment region (S/MAR) DNA and can function as an estrogen receptor corepressor and can also inhibit cell proliferation.
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References
- Zheng B, Zhou Q, Guo Y. Establishment of a proteomic profile associated with gonocyte and spermatogonial stem cell maturation and differentiation in neonatal mice. Proteomics. 14(2-3):274-85. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"