S6 Phosphate Acceptor Peptide, S6-1

S6 Phosphate Acceptor Peptide, S6-1

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

RRLSSLRA

H-Arg-Arg-Leu-Ser-Ser-Leu-Arg-Ala-OH

8

97.1%

C₃₉H₇₅N₁₇O₁₁

958.13

93674-74-9

Synthetic

Powder

This peptide is based on the sequence of a region of rat hepatic ribosomal protein S6 which contains the major phosphorylation site for cAMP-dependent protein kinase and one of the insulin-stimulated phosphorylation sites. It has been shown to be a substrate for cAMP-dependent protein kinase, for protein kinase C, and for the ribosomal protein S6 kinases (p70S6K and p85S6K isoforms, as well as insulin-stimulated p90rsk).

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• B.Gabrielli et al., FEBS Lett., 175, 219 (1984)
• S.Ferrari et al., FEBS Lett., 184, 72 (1985)
• S.L.Pelech et al., Proc. Natl. Acad. Sci. USA, 83, 5968 (1986)
• H.Flotow and G.Thomas, J. Biol. Chem., 267, 3074 (1992)
• D.E.Joseph et al., J. Biol. Chem., 271, 13088 (1996)

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.