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Product Name
RYR2 Polyclonal Antibody
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Description
Polyclonal antibody to RYR2
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
RYR2 antibody; ARVC2 antibody; ARVD2 antibody; RYR-2 antibody; RyR antibody; VTSIP antibody; ryanodine receptor 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 4850-4950 of human RYR2 (NP_001026.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - RYR2 Polyclonal Antibody
Western blot analysis of extracts of mouse heart, using RYR2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
Immunohistochemistry - RYR2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using RYR2 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - RYR2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human placenta using RYR2 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - RYR2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using RYR2 Antibody at dilution of 1:100 (40x lens).
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Background
Calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering cardiac muscle contraction. Aberrant channel activation can lead to cardiac arrhythmia. In cardiac myocytes, calcium release is triggered by increased Ca(2+) levels due to activation of the L-type calcium channel CACNA1C. The calcium channel activity is modulated by formation of heterotetramers with RYR3. Required for cellular calcium ion homeostasis. Required for embryonic heart development.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"