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Product Name
RTN1 (Isoform RTN1-C) antibody
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Description
RTN1 (Isoform RTN1-C) Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, human brain tissue. Positive IHC detected in human brain tissue, human placenta tissue. Observed molecular weight by Western-blot: 23 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
NSP antibody; NSP-C antibody; reticulon 1 antibody; RTN1 antibody; RTN1-C antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RTN1 (Isoform RTN1-C) recombinant protein (Accession Number: NM_206852). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114861(RTN1 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human brain using Catalog No:114861(RTN1 antibody) at dilution of 1:100 (under 40x lens)
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Background
Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. Human RTN1 (also known as NSP) gene is expressed predominantly in neuroendocrine tissues, and produces three isoforms termed RTN1-A (NSP-A), RTN1-B (NSP-B), and RTN1-C (NSP-C). This antibody is raised against the full-length protein of human RTN1-C.
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