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Product Name
RPS6 antibody
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Description
RPS6 Rabbit Polyclonal antibody. Positive WB detected in K-562 cells, MCF7 cells. Positive IP detected in MCF-7 cells. Observed molecular weight by Western-blot: 29 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
40S ribosomal protein S6 antibody; Phosphoprotein NP33 antibody; ribosomal protein S6 antibody; RPS6 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RPS6 recombinant protein (Accession Number: NM_001010). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:114911(RPS6 antibody) at dilution of 1:500
IP Result of anti-RPS6 (IP:Catalog No:114911, 4ug; Detection:Catalog No:114911 1:400) with MCF-7 cells lysate 1040ug.
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Background
Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.It's molecular weight is about 29kd.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"