-
Product Name
RPS4X antibody
- Documents
-
Description
RPS4X Rabbit Polyclonal antibody. Positive IP detected in HepG2 cells. Positive WB detected in HeLa cells, HepG2 cells, human liver tissue, MCF7 cells, mouse pancreas tissue. Positive IHC detected in human pancreas cancer tissue. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 30 kDa
-
Tested applications
ELISA, WB, IHC, IF, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
CCG2 antibody; DXS306 antibody; FLJ40595 antibody; ribosomal protein S4 antibody; X linked antibody; RPS4 antibody; RPS4X antibody; SCAR antibody; SCR10 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of RPS4X recombinant protein (Accession Number: NM_001007). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
-
Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114908(SCAR antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:114908(SCAR antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:114908(SCAR antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using RPS4X antibody Catalog No:114908 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-SCAR (IP:Catalog No:114908, 3ug; Detection:Catalog No:114908 1:500) with HepG2 cells lysate 3200ug.
-
Background
RPS4X, a X-linked gene in the human, encodes a subunit of ribosomal protein S4, a component of the 40S subunit and 60S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene, RPS4Y2 and the ribosomal protein S4, X-linked (RPS4X). [PMID:8358435]
-
References
- Tsofack SP, Meunier L, Sanchez L. Low expression of the X-linked ribosomal protein S4 in human serous epithelial ovarian cancer is associated with a poor prognosis. BMC cancer. 13:303. 2013.
- Paquet ÉR, Hovington H, Brisson H. Low level of the X-linked ribosomal protein S4 in human urothelial carcinomas is associated with a poor prognosis. Biomarkers in medicine. 9(3):187-97. 2015.
- Mino T, Murakawa Y, Fukao A. Regnase-1 and Roquin Regulate a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms. Cell. 161(5):1058-73. 2015.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"