-
Product Name
RPS3A Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to RPS3A
-
Tested applications
WB, IHC, IF, IP
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
RPS3A antibody; FTE1 antibody; MFTL antibody; S3A antibody; ribosomal protein S3A antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human RPS3A (NP_000997.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100 -
Validations
Western blot - RPS3A Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using RPS3A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - RPS3A Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using RPS3A Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - RPS3A Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver cancer using RPS3A Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - RPS3A Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using RPS3A Antibody at dilution of 1:100 (40x lens).
Immunofluorescence - RPS3A Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using RPS3A antibody . Blue: DAPI for nuclear staining.
Immunoprecipitation - RPS3A Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of MCF7 cells using 3ug RPS3A antibody . Western blot was performed from the immunoprecipitate using RPS3A antibody at a dilition of 1:500.
-
Background
May play a role during erythropoiesis through regulation of transcription factor DDIT3.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"