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Product Name
RPS3 antibody
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Description
RPS3 Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, COLO 320 cells, HeLa cells, mouse colon tissue, NIH/3T3 cells, rat brain tissue. Positive IP detected in mouse brain tissue. Positive IHC detected in human ovary tumor tissue, human colon cancer tissue. Observed molecular weight by Western-blot: 33 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
40S ribosomal protein S3 antibody; FLJ26283 antibody; FLJ27450 antibody; ribosomal protein S3 antibody; RPS3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RPS3 recombinant protein (Accession Number: NM_001256802). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114844(RPS3 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:114844(RPS3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:114844(RPS3 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-RPS3 (IP:Catalog No:114844, 4ug; Detection:Catalog No:114844 1:1000) with mouse brain tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:114844(RPS3 Antibody) at dilution of 1:50 (under 40x lens)
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Background
The ribosomal protein S3 is a subunit of 40S ribosomal protein, and has two distinct function. RPS3 acts as a ribosome protein that contributes to the domain of the ribosome where translation is initiated, and involves in repair of UV damage as an endonuclease. Besides binding RNA within the 40S ribosomal subunit, RPS3 participates in transcription, and apoptosis.
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References
- Faza MB, Chang Y, Occhipinti L, Kemmler S, Panse VG. Role of Mex67-Mtr2 in the nuclear export of 40S pre-ribosomes. PLoS genetics. 8(8):e1002915. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"