-
Product Name
RPS27 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to RPS27
-
Tested applications
WB, IHC
-
Species reactivity
Human, Rat
-
Alternative names
RPS27 antibody; MPS-1 antibody; MPS1 antibody; S27 antibody; ribosomal protein S27 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-84 of human RPS27 (NP_001021.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - RPS27 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using RPS27 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - RPS27 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using RPS27 Antibody at dilution of 1:100 (40x lens).
-
Background
Component of the small ribosomal subunit. Required for proper rRNA processing and maturation of 18S rRNAs.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"