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Product Name
RPS25 antibody
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Description
RPS25 Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive IHC detected in human breast cancer tissue, human liver tissue. Positive WB detected in Jurkat cells, A549 cells, HeLa cells, K-562 cells, MCF7 cells. Observed molecular weight by Western-blot: 15-17 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
40S ribosomal protein S25 antibody; ribosomal protein S25 antibody; RPS25 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RPS25 recombinant protein (Accession Number: NM_001028). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:114836(RPS25 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human liver slide using Catalog No:114836(RPS25 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human breast cancer slide using Catalog No:114836(RPS25 Antibody) at dilution of 1:50
Immunofluorescent analysis of MCF-7 cells using Catalog No:114836(RPS25 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Ribosomal protein S25 (RPS25) is a novel MDM2 interacting protein. It may be involved in viral replication of Dicistroviridae and hepatitis C viruses. RPS25 is overexpressed in human leukemia cells exhibiting adriamycin resistance. It may have a role in p53-mediated apoptosis and cell-cycle arrest. The expected MW of RPS25 is about 15-17kDa. Catalog# 23599-1-AP is a rabbit polyclonal antibody raised against full-length RPS25, and is recommended for ELISA, Western blotting and IP assays.
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