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Product Name
RPS19 antibody
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Description
RPS19 Rabbit Polyclonal antibody. Positive WB detected in human testis tissue, HEK-293 cells, mouse testis tissue, rat colon tissue. Positive IP detected in HEK-293 cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 16kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
40S ribosomal protein S19 antibody; DBA antibody; ribosomal protein S19 antibody; RPS19 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RPS19 recombinant protein (Accession Number: NM_001022). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:114832(RPS19 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114832(RPS19 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114832(RPS19 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-RPS19 (IP:Catalog No:114832, 4ug; Detection:Catalog No:114832 1:500) with HEK-293 cells lysate 880ug.
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Background
The mammalian ribosome consists of 4 RNA species and approximately 80 different proteins. The ribosomal proteins are encoded by complex gene families that include at least 1 active intron-containing gene and multiple processed pseudogenes. The RPS19 protein is a component of the 40S ribosomal subunit [PMID: 17726054]. Diamond-Blackfan anemia (DBA) is a congenital erythroid hypoplasia caused by a functional haploinsufficiency of genes encoding for ribosomal proteins. Among these genes, ribosomal protein S19 (RPS19) is mutated most frequently [PMID:21989989]. It also a p53-related ribosomal protein that possibly involves cellular apoptosis through the BAX/p53 pathway [PMID:22272377].
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References
- Acquadro E, Caron I, Tortarolo M, Bucci EM, Bendotti C, Corpillo D. Human SOD1-G93A specific distribution evidenced in murine brain of a transgenic model for amyotrophic lateral sclerosis by MALDI imaging mass spectrometry. Journal of proteome research. 13(4):1800-9. 2014.
- Armistead J, Hemming R, Patel N, Triggs-Raine B. Mutation of EMG1 causing Bowen-Conradi syndrome results in reduced cell proliferation rates concomitant with G2/M arrest and 18S rRNA processing delay. BBA clinical. 1:33-43. 2014.
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