RPN1 Polyclonal Antibody

Cat.#: 165003

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Product Information

  • Product Name
    RPN1 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to RPN1
  • Tested applications
    WB, IHC, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    RPN1 antibody; OST1 antibody; RBPH1 antibody; ribophorin I antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human RPN1 (NP_002941.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:100
    IF 1:50 - 1:100
  • Validations

    Western blot - RPN1 Polyclonal Antibody

    Western blot - RPN1 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using RPN1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

    Immunohistochemistry - RPN1 Polyclonal Antibody

    Immunohistochemistry - RPN1 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded rat kidney using RPN1 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - RPN1 Polyclonal Antibody

    Immunohistochemistry - RPN1 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded human liver cancer using RPN1 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - RPN1 Polyclonal Antibody

    Immunohistochemistry - RPN1 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded human liver injury using RPN1 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - RPN1 Polyclonal Antibody

    Immunohistochemistry - RPN1 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded human gastric cancer using RPN1 antibody at dilution of 1:100 (40x lens).

  • Background
    Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"