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Product Name
RPN1 Polyclonal Antibody
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Description
Polyclonal antibody to RPN1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
RPN1 antibody; OST1 antibody; RBPH1 antibody; ribophorin I antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human RPN1 (NP_002941.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100 -
Validations
Western blot - RPN1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using RPN1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - RPN1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using RPN1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - RPN1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver cancer using RPN1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - RPN1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver injury using RPN1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - RPN1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric cancer using RPN1 antibody at dilution of 1:100 (40x lens).
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Background
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"