-
Product Name
RPL5 antibody
- Documents
-
Description
RPL5 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, human liver tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human liver cancer tissue, human kidney tissue. Observed molecular weight by Western-blot: 34 kDa
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
60S ribosomal protein L5 antibody; DBA6 antibody; MSTP030 antibody; ribosomal protein L5 antibody; RPL5 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of RPL5 recombinant protein (Accession Number: BC001882). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
-
Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:114897(RPL5 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:114897(RPL5 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:114897(RPL5 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:114897(RPL5 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
The mammalian ribosome is a macromolecular assembly of 4 RNA species (rRNAs) and approximately 80 different proteins, including RPL5. RPL5 （ribosome protein L15） is rquired for rRNA maturation and formation of the 60S ribosomal subunits, and defects or mutation in RPL5 is the cause of DBA6.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"