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Product Name
RPL3 antibody
- Documents
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Description
RPL3 Mouse Monoclonal antibody. Positive WB detected in HEK-293 cells, HeLa cells, HepG2 cells. Positive IHC detected in human lung cancer tissue, human placenta tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 46 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
60S ribosomal protein L3 antibody; ribosomal protein L3 antibody; RPL3 antibody; TARBP B antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of RPL3 recombinant protein (Accession Number: NM_000967). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:107509(RPL3 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human lung cancer slide using Catalog No:107509(RPL3 Antibody) at dilution of 1:50
Immunofluorescent analysis of HepG2 cells using Catalog No:107509(RPL3 Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG
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Background
Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL3, also named as TARBP-B and MGC104284, belongs to the ribosomal protein L3P family. This protein is a component of the 60S subunit. It can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"