RPL3 antibody

Cat.#: 107509

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Product Information

  • Product Name
    RPL3 antibody
  • Documents
  • Description
    RPL3 Mouse Monoclonal antibody. Positive WB detected in HEK-293 cells, HeLa cells, HepG2 cells. Positive IHC detected in human lung cancer tissue, human placenta tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 46 kDa
  • Tested applications
    ELISA, WB, IHC, IF
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    60S ribosomal protein L3 antibody; ribosomal protein L3 antibody; RPL3 antibody; TARBP B antibody
  • Immunogen
  • Isotype
    Mouse IgG1
  • Preparation
    This antibody was obtained by immunization of RPL3 recombinant protein (Accession Number: NM_000967). Purification method: Protein G purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:107509(RPL3 antibody) at dilution of 1:1000

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:107509(RPL3 antibody) at dilution of 1:1000

    Immunohistochemistry of paraffin-embedded human lung cancer slide using Catalog No:107509(RPL3 Antibody) at dilution of 1:50

    Immunohistochemistry of paraffin-embedded human lung cancer slide using Catalog No:107509(RPL3 Antibody) at dilution of 1:50

    Immunofluorescent analysis of HepG2 cells using Catalog No:107509(RPL3 Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG

    Immunofluorescent analysis of HepG2 cells using Catalog No:107509(RPL3 Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG

  • Background
    Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL3, also named as TARBP-B and MGC104284, belongs to the ribosomal protein L3P family. This protein is a component of the 60S subunit. It can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

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