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Product Name
RPL23 antibody
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Description
RPL23 Rabbit Polyclonal antibody. Positive IHC detected in human brain. Positive WB detected in HeLa cells, PC-3 cells. Observed molecular weight by Western-blot: 15kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
RPL23 antibody; rpL17 antibody; 60S ribosomal protein L17 antibody; ribosomal protein L23 antibody; 60S ribosomal protein L23 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RPL23 recombinant protein (Accession Number: NM_000978). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114878(RPL23 antibody) at dilution of 1:200
Immunohistochemical of paraffin-embedded human brain using Catalog No:114878(RPL23 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:114878(RPL23 antibody) at dilution of 1:50 (under 40x lens)
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Background
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins [PMID:9582194]. RPL23 is a component of the 60 S large ribosomal subunit that interacts functionally with murine double minute 2 protein (MDM2 or HDM2 in humans). Upon binding to MDM2, RPL23 inhibits MDM2-mediated p53 degradation, thus activating p53 and inducing cellular apoptosis [PMID:15314174]. Intriguingly, RPL23 has also been suggested to be a negative regulator of apoptosis by suppressing the Miz1-induced transcription of the cell cycle inhibitors p15INK4B and p21CIP1[PMID:19160485].
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References
- Wong CC, Traynor D, Basse N, Kay RR, Warren AJ. Defective ribosome assembly in Shwachman-Diamond syndrome. Blood. 118(16):4305-12. 2011.
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