Product Name

RPL13 Polyclonal Antibody

Documents

Description

Polyclonal antibody to RPL13

Tested applications

WB, IHC, IF

Species reactivity

Human, Mouse, Rat

Alternative names

RPL13 antibody; BBC1 antibody; D16S444E antibody; D16S44E antibody; L13 antibody; ribosomal protein L13 antibody

Isotype

Rabbit IgG

Preparation

Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human RPL13 (NP_150254.1).

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage instructions

Store at -20℃. Avoid freeze / thaw cycles.

Applications

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Validations

Western blot - RPL13 Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using RPL13 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.

Western blot - RPL13 Polyclonal Antibody

Western blot analysis of extracts of various cells, using RPL13 antibody at 1:1000 dilution ratio through one-step method.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:4000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.