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Product Name
RPAP1 antibody
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Description
RPAP1 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, HEK-293 cells, human testis tissue, mouse brain tissue, SH-SY5Y cells. Observed molecular weight by Western-blot: 153 kDa
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Tested applications
ELISA, IP, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp727M111 antibody; FLJ12732 antibody; KIAA1403 antibody; RPAP1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RPAP1 recombinant protein (Accession Number: NM_015540). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114797(RPAP1 antibody) at dilution of 1:800
IP Result of anti-RPAP1 (IP:Catalog No:114797, 3ug; Detection:Catalog No:114797 1:800) with HeLa cells lysate 3000ug.
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Background
RNA polymerase II-associated protein 1 (RPAP1) encodes a nuclear 153-kDa protein, and forms an interface between the RNA polymerase II (RNAPII) enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. through its interaction with acetylated histones. RPAP1 is required for interaction of the RNA polymerase II complex with acetylated histone H3 and the subsequent DNA transcription.
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