ROBO3 Polyclonal Antibody

Cat.#: 162342

Special Price 174.2 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    ROBO3 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to ROBO3
  • Tested applications
    WB
  • Species reactivity
    Human
  • Alternative names
    ROBO3 antibody; HGPPS antibody; HGPS antibody; RBIG1 antibody; RIG1 antibody; roundabout homolog 3 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human ROBO3 (NP_071765.2).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
  • Validations

    Western blot - ROBO3 Polyclonal Antibody

    Western blot - ROBO3 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using ROBO3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.

  • Background
    Thought to be involved during neural development in axonal navigation at the ventral midline of the neural tube. In spinal chord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1 (By similarity). Required for hindbrain axon midline crossing.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"